Biosynthesis of resveratrol derivatives and evaluation of their anti-inflammatory activity

Abstract Resveratrol is a typical plant phenolic compound whose derivatives are synthesized through hydroxylation, O -methylation, prenylation, and oligomerization. its exhibit anti-neurodegenerative, anti-rheumatoid, anti-inflammatory effects. Owing to the diverse biological activities of these compounds their importance in human health, this study attempted synthesize five resveratrol (isorhapontigenin, pterostilbene, 4-methoxyresveratrol, piceatannol, rhapontigenin) using Escherichia coli . Two-culture system was used improve final yield derivatives. first E. cell that harbored genes for biosynthesis including TAL (tyrosine ammonia lyase), 4CL (4-coumaroyl CoA ligase), STS (stilbene synthase) tyrosine such as aroG (deoxyphosphoheptonate aldolase) tyrA (prephenate dehydrogenase). Thereafter, culture filtrate from modification reaction carried out second harboring hydroxylase and/or -methyltransferase. Approximately, 89.8 mg/L same, were prepared with conversion rate 88.2% 22.9%. Using derivatives, we evaluated activity. 4-Methoxyresveratrol, pterostilbene isorhapontigenin showed effects without any toxicity. In addition, exhibited enhanced macrophages compared resveratrol.